Figure 1. Effects of Sema3A on proliferation and vascular endothelial growth factor (VEGF) secretion of human umbilical vein endothelial cells (HUVECs). A: The panel is the statistical results for HUVECs proliferation at 24 h time point. B: The panel is the statistical results for HUVECs proliferation at 48 h time point. C: The panel is the statistical results for HUVECs proliferation at 72 h time point. In panel A, B and C, the y-axis represents the proliferation ratio that different treatment groups comparing to 10% FBS treatment group, and each experiments were repeated at least three times. D: The panel is the statistical results for free VEGF165 secretion as measured by an enzyme-linked immuno sorbent assay (ELISA) at 24 h time point. E: The panel is the statistical results for free VEGF165 secretion as measured by an ELISA assay at 48 h time point. F: The panel is the statistical results for free VEGF165 secretion as measured by an ELISA assay at 72 h time point. In panel D, E and F, the y-axis represents the value the detected by ELISA Kit and each experiment were repeated at least three times. All of the data were presented as mean± standard error of the mean (SEM). *p<0.05; **p<0.01.