Molecular Vision: Schlamp, Mol Vis 2013; 19:1387-1396. Table 2

Table 2. Summary of ganglion cell percentages with different labeling paradigms

Method	Number Retinas Analyzed	Total Fields Counted	RGC Percentage (mean ± SD)	Range
FG_r-DAPI_r	6 (3 mice)	218	53.51±17.10%	94% to 20%
FG_r-DAPI_r	2 (2 rats)	70	50.21±12.66%	84% to 23%
FG_r-TO-PRO-3_cs	11 (7 mice)	138	49.10±11.88%	78% to 20%
DiI_r-DAPI_cs	6 (6 mice)	57	51.14±9.68%	71% to 33%
NeuN-DAPI_cs	4 (2 mice)	16	68.33±5.51%	80% to 62%
BRN3A-DAPI_cs	4 (2 mice)	30	44.76±9.13%	65% to 29%
BRN3B-DAPI_cs	4 (2 mice)	30	54.05±12.79%	79% to 31%
BRN3 combined	4 (2 mice)	30	61.33±13.9%	87% to 37%

The method column refers to the methods use to identify both retinal ganglion cells and the nuclear stain for estimating total neurons. Retrograde dyes are indicated with a subscript “r,” while counter-stains are represented with a subscript “cs.” The data designated as “BRN3 combined” represents all cells that were positive for BRN3A + BRN3B in the same field. Approximately 61% of these cells were positive for both BRN3 proteins. A single field represents an area of 10⁴ µm², and contained approximately 100 neurons.