Table 1 of
Sousounis, Mol Vis 2013; 19:135-145. Table 1 of
Sousounis, Mol Vis 2013; 19:135-145.
Table 1. List of primers for genes tested by qRT-PCR and annealing temperatures used for their respective target genes.
| Genes | Annealing temperature – band size |
|---|---|
| RPL27 | 55 °C - ~100 bp |
| Forward 5′ – 3′ | ATTTATGAAACCCGGGAAGG |
| Reverse 5′ – 3′ | CCAGGGCATGACTGTAAGGT |
| GAPDH | 55 °C – 509 bp |
| Forward 5′ – 3′ | GCCTCCTGTACTACCAACTG |
| Reverse 5′ – 3′ | CCCCACTCGTTGTCATACCA |
| Cytochrome c oxidase subunit 2 | 61 °C – 355 bp |
| Forward 5′ – 3′ | ACACTAACGCAATAGACGCACAAGA |
| Reverse 5′ – 3′ | ACGCCCATTGAGGGGACTGC |
| Rad1 | 61 °C – 341 bp |
| Forward 5′ – 3′ | TGCGTGCCTCGACAACGTCC |
| Reverse 5′ – 3′ | TCACCACCCCACCTTCCTCCA |
| Stromelysin 1/2 a | 61 °C – 168 bp |
| Forward 5′ – 3′ | GGGGGACAAAGACTCTCCCCGA |
| Reverse 5′ – 3′ | CTGGTGTTCTTCAGTGTCCGGGT |
| VEGFR1 | 61 °C – 227 bp |
| Forward 5′ – 3′ | TCCTGCAGCAGCCTGACCTTG |
| Reverse 5′ – 3′ | GTTTGGGGCTGTGACTCGGC |
| Avidin | 60 °C – 103 bp |
| Forward 5′ – 3′ | TCGTTTCTCCTCTGACGGGCT |
| Reverse 5′ – 3′ | TGCCCTGCCCAGGTGGTGAT |
| Glutathione peroxide 1 | 60 °C – 104 bp |
| Forward 5′ – 3′ | GCTGGTGGTGCTGGGCTTCC |
| Reverse 5′ – 3′ | ACCCTTTTCCTGGACGAACGTACT |