Table 2 of
Strzalka-Mrozik, Mol Vis 2013; 19:1341-1349. Table 2 of
Strzalka-Mrozik, Mol Vis 2013; 19:1341-1349.
Table 2. Characteristics of primers used for real-time QRT-PCR
| Gene | Sequence of primers | Length of amplicon (bp) * | Tm (°C) † |
|---|---|---|---|
| SOD2 | Forward: 5′-CTGATTTGGACAAGCAGCAA-3′ | 199 | 81.6 |
| Reverse: 5′-CTGGACAAACCTCAGCCCTA-3′ | |||
| ALDH1A1 | Forward: 5′-TACTCACCGATTTGAAGATT-3′ | 151 | 77.2 |
| Reverse: 5′-TTGTCAACATCCTCCTTATC-3′ | |||
| MGST1 | Forward: 5′- ATTGGCCTCCTGTATTCCTTG-3′ | 311 | 80.2 |
| Reverse: 5′-TAATCCCTCTGCTCCCCTCC-3′ | |||
| GAPDH | Forward: 5′-GAAGGTGAAGGTCGGAGTC-3′ | 226 | 80.0 |
| Reverse: 5′-GAAGATGGTGATGGGATTC-3′ |