Figure 6. Effects of PI3K-Akt, ERK1/2, p38 blockade in porcine trabecular meshwork (PTM) cells treated with medium alone or 600 μM H₂O₂ for various amounts of time. PTM (1x10⁵) cells were seeded into 12-well plates. Three days later, the medium was changed to serum-free medium overnight. PTM cells were pretreated with vehicle (A), LY294002 (10 μM; B), U0126 (10 μM; C), SB203580 (10 μM; D), inhibitors of PI3K, ERK1/2, or p38, respectively, for 1 h, and then treated with medium alone (A) or 600 μM H₂O₂ (B–D) for various amounts of time. The cells were stained with propidium iodide to detect cell death, (red; middle panel). Nuclei were stained with Hoechst 33342 (blue; lower panel).