Figure 2. Effects of small interfering RNA–targeting tissue factor on tissue factor knockdown in the in vitro model of neovascularization. A: Tissue factor (TF) messenger RNA (mRNA) expression levels in human umbilical vein endothelial cells (HUVECs) after lipopolysaccharide (LPS) stimulation at 24 h, after 5 h of exposure to various small interfering RNAs (siRNAs). Relative quantification of TF mRNA expression is shown as mean±standard deviation (SD) of three independent experiments; *, p<0.01. B: Representative western blots show the TF protein expression levels at 24 h, after 5 h exposure to various siRNAs. β-actin was the internal loading control.