Figure 2 of Liu, Mol Vis 2013; 19:1268-1281.


Figure 2. Disease-associated mutations in CNGB3 increase cytotoxicity. A: Western blot demonstrating expression of FLAG-tagged wild-type (WT) and mutant cone CNG channel subunits in 661W cells following transfection with indicated plasmids (above). Approximate locations of molecular weight markers (in kilodaltons) are indicated to the right of the immunoblot. Cell lysates were also probed with beta-actin antibody (below). The molecular weight of beta actin was ~42 kDa. B: The bar graph demonstrates increased cytotoxicity (measured as LDH release from dying cells) for cells expressing CNGA3 plus wild-type (WT) or mutant CNGB3 exposed to various concentrations of CPT-cGMP for 24 h (n=46 to 48). Cytotoxicity was normalized to that of control cells transfected with vector (pOPRSVI) alone, incubated in the absence of CPT-cGMP; *, significant difference between groups indicated by bracket (p<0.05); +, significant difference between F525N groups with or without 0.1 μM CPT-cGMP treatment (p<0.01).