Table 1 of
Karri, Mol Vis 2013; 19:1231-1237. Table 1 of
Karri, Mol Vis 2013; 19:1231-1237.
Table 1. List of primers used for cloning and sequencing HGSC and its D26G mutant.
| Clone | Primer | Primer sequence |
|---|---|---|
| pET21-a-γS | Forward | 5′GGGAGTTCCATATGTCTAAAACTGGAACC3′ |
| Reverse | 5′CCGGAATTCTTACTCCACAATGCG3′ | |
| pET21-a-γSD26G | Forward | 5′CTATGACTGTGATTGCGGCTGTGCAGATTTCCACACATAC 3′ |
| Reverse | 5′GAAATCTGCACAGCCGCAATCACAGTCATAGCGAC 3′ | |
| T7 | Forward | 5′TAATACGACTCACTATAGG3′ |
| T7 | Reverse | 5′TATGCTAGTTATTGCTCAG3′ |
| BGH | Reverse | 5′TAGAAGGCACAGTCGAGG3′ |