Figure 6. Results of the terminal deoxynucleotidyl transferase-mediated nick end labeling assay. A: Apoptosis (red color) was observed only in a few cells in the normal cornea. B: In a cornea from group A, apoptosis was detected mainly in the posterior stroma and endothelial layer. C: In a cornea from group B, severe apoptosis throughout all layers of the cornea occurred. Scale bar=50 μm. *taken from the opposite eye that did not undergo cryoinjury.