Table 2 of
Gong, Mol Vis 2013; 19:121-127. Table 2 of
Gong, Mol Vis 2013; 19:121-127.
Table 2. Primers used for mutation screening in MMP2 gene.
| Exon | Primer sequence(5′-3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| 1 | F: GTACTGTGCCATCCTAAT | 445 | 56 |
| R: CTGTCTGACTTCATTTTCT | |||
| 2 | F: CACATACACGCAGGCACA | 614 | 62 |
| R: CCATATTGGACAGCACAGT | |||
| 3 and 4 | F: TTTCAGGGTCTAGGTGGC | 677 | 62 |
| R: GGAACTGAGTGAAGGACG | |||
| 5 | F: GAGAAGCAGCTCCTTACCA | 463 | 59 |
| R: GGATGTCATTCGCACAGAT | |||
| 6 | F: AGCGTCATGTCATTGCTT | 378 | 62 |
| R: CTGGGTAGGTGGGTGTCT | |||
| 7 | F: ACAAGAAGACTTTGGCTGAC | 595 | 59 |
| R: TTCGGATAGGGAAGAGTTA | |||
| 8 | F: AGAGGACTGATTTGGGTGAT | 404 | 62 |
| R: GGACAGGAGACAAGGAGG | |||
| 9 | F: CAGGGTAGGAGGATGTTTC | 561 | 61 |
| R: AATGCTATCTGATGTTGGGT | |||
| 10 | F: TGACTTCTAAAGCCCTCTG | 375 | 59 |
| R: AACTGTGCTGCTGTCCTAC | |||
| 11 | F: AAGGGCTAGGTCCAGTTTC | 414 | 59 |
| R: CAAGGAGCAGAGGTCAGG | |||
| 12 | F: TGGGCTCAAGCAATCCTC | 309 | 59 |
| R: TGTATCGAAGGCAGTGGA |