Figure 3. Photoreceptor-like cells in ARPE-19 cultures transfected with adeo-associated viral DNA expressing human neurogenin1. Shown are morphologies of cells in a control culture transfected with adeo-associated viral (AAV) DNA expressing GFP (AAV-GFP;
A) and in an experimental culture transfected AAV DNA expressing human neurogenin1 (AAV-ngn1: B). Arrows point to cells with neuron-like morphologies, and arrowheads point to cells maintaining the morphologies of ARPE-19
cells. C-H are immunofluorescent detection of photoreceptor proteins arrestin (Arr), recoverin (Rcv) and transducin β-subunit (GβT)
in ARPE-19 cell cultures transfected with AAV-GFP (A-C) or AAV-ngn1 (D-F). Scale bar (50 μm) applies to all panels.