Figure 2. Expression of photoreceptor proteins in hTERT-RPE1 cultures transfected (by electroporation) with MSCV retroviral DNA expressing
control GFP or experimental gene human neurogenin1. Shown are immunofluorescent detection in the control cultured transfected with MSCV DNA expressing control GFP (MSCV-GFP)
for retinal cone arrestin 3 (Arr; A), recoverin (Rcv; B), and red opsin (Red; C) and in the experimental culture transfected with MSCV DNA expressing human neurogenin1 (MSCV-ngn1) of each protein (D-F). Scale bars are 50 μm.