Figure 1. Detection photoreceptor gene expression in cultures of hTERT-RPE1 cells infected with retroviral MSCV expressing control GFP
or experimental gene neuroD. Shown are in situ mRNA hybridization for neuroD mRNA (A, B), retinal cone arrestin 3 mRNA (Arr; C, D), recoverin mRNA (Rcv, E, F), and red opsin mRNA (Red; G, H), in the two sets of cultures with A, C, E, G representing the control. I-K are in situ hybridization detection for mRNA of interphotoreceptor retinoid binding protein
(IRBP; I), the γ-subunit of phosphodiesterase (γ-PDE; J), and cone-rod homeobox protein (Crx; K) of hTERT-RPE1 cell culture infected with MSCV retrovirus expressing neuroD. Scale bar (20 µm) applies to all panels.