Figure 7. Confirmation of the change in the paired box 6 gene messenger ribonucleic acid expression in patients with aniridia and unaffected members of the family using real-time polymerase chain reaction. The paired box 6 (PAX6) gene messenger ribonucleic acid (mRNA) level (normalized to glyceraldehyde-3-phosphate dehydrogenase) was about 50% lower in patients with aniridia than in unaffected members (*p<0.01).