Table 4 of Shei, Mol Vis 2013; 19:1096-1106.

Table 4. Calculation of normalization factors for most stable reference genes

Cell source 18s rRNA Actb Hprt1 Norm. Factor
Cultured MCEC (passage 2) 1.00 1.00 1.00 1.00
Cultured MCEC (passage 7) 0.14 0.27 0.32 0.2313
Primary corneal endothelium 0.76 0.34 0.37 0.4564
M <1.5 1.323 0.973 0.963

The geometric means of the most stable reference genes were calculated to obtain the normalization factor. MCEC, mouse corneal endothelial cells; 18s rRNA, 18s ribosomal RNA; Actb, β actin; Hprt1, Hypoxanthine phosphoribosyltransferase 1; Norm factor, normalization factor.

Shei, Mol Vis 2013; 19:1096-1106. http://www.molvis.org/molvis/v19/1096

©2013 Molecular Vision http://www.molvis.org/molvis/

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