Figure 1. Analysis of Slc4 gene expression by reverse transcription polymerase chain reactions (RT–PCR). Template cDNA samples were generated from mouse primary corneal endothelium (A) and cultured mouse corneal endothelial cells at passage 2 (B) and at passage 7 (C). A 100 base pair (bp) marker is shown in the first lane from the left. The primers used to generate the PCR products (Table 1) are indicated below each lane. Four housekeeping genes (Gapdh, Actb, Hprt1, and 18s rRNA) were used as amplification (positive) controls. Each set of reactions (per gene) included a no-template (NT) negative control; these samples were pooled and the combined sample was subject to electrophoresis.