Figure 8. Each of the three nuclear layers viewed under the fundus lens. A: View of a transgenic fish eye with green fluorescent protein (GFP) in the retinal ganglion cells. B: The vessels of the inner eye partially labeled with lectin peanut agglutinin (PNA; arrows indicate edges of a vessel) as an alternative to using the fli1a:EGFP line. C: View of putative bipolar cells of the INL in a fish of the Et(fos:Gal4-VP16)s1181t; Tg(UAS-E1b:Kaede)s1999t line. See Appendix 4 to view this line in vivo. D: Example cryosection of the eye visualized in C, chosen to show bipolar cell morphology near the optic nerve head. E: Same as D, with nuclear stain. The location of the Kaede-labeled cells was confirmed to be the INL, near the ONH. F: Higher magnification view of inset in image D, showing bipolar morphology. Apical and basal extensions to left and right are consistent with bipolar cell morphology. ONH: optic nerve head; ONL: outer nuclear layer; INL: inner nuclear layer. Video of the INL can be found online in Appendix 4. n=3 fish shown.