Figure 6. Alterations in the levels of total O-GlcNAc modified proteins in retinal pericytes (PC) by high glucose and specific inhibitors for glutamine fructose-6-phosphate amidotransferase (GFAT), O-GlcNAc transferase (OGT), or O-GlcNAcase (OGA). A: Protein lysates (50 µg) from retinal vascular cells were analyzed by western blot analysis for O-GlcNAcylated proteins under 5 mM and 25 mM glucose with or without inhibitors. Thiamet-G and PUGNAc are OGA inhibitors. DON is a GFAT inhibitor and Alloxan is an OGT inhibitor. B: The β-actin expression was assessed as a loading control and used for normalization and quantification of data obtained from three different runs. Mean±SEM; *** (p≤0.001) significantly different from 5 mM glucose control.