Figure 2. Increased O-GlcNAcylation and decreased O-GlcNAcase (OGA) expression in the retinas of Ins2^Akita/+ mice. O-GlcNAc transferase (OGT) expression did not show any alterations. A: Protein lysates (25 µg) from wild-type C57BL/6J and Ins2^Akita/+ mice retina were examined by western blot analysis for O-GlcNAcylated proteins and expression of OGT and OGA. B: The β-actin expression was assessed as a loading control and used for normalization and quantification of data, which were obtained after three different runs. C: Increases in the RNA expression of OGT and OGA were detected in Ins2^Akita mice retina by qPCR and normalized to RpL13A RNA expression. The qPCRs were performed with three biologic replicates and in triplicate. Mean±SEM; * (p≤0.05), ** (p≤0.01), and ***(p≤0.001) significantly different from wild-type mice at the same time points of wild-type.