Figure 2. Increased O-GlcNAcylation and decreased O-GlcNAcase (OGA) expression in the retinas of Ins2Akita/+ mice. O-GlcNAc transferase (OGT) expression did not show any alterations. A: Protein lysates (25 µg) from wild-type C57BL/6J and Ins2Akita/+ mice retina were examined by western blot analysis for O-GlcNAcylated proteins and expression of OGT and OGA. B: The β-actin expression was assessed as a loading control and used for normalization and quantification of data, which were
obtained after three different runs. C: Increases in the RNA expression of OGT and OGA were detected in Ins2Akita mice retina by qPCR and normalized to RpL13A RNA expression. The qPCRs were performed with three biologic replicates and
in triplicate. Mean±SEM; * (p≤0.05), ** (p≤0.01), and ***(p≤0.001) significantly different from wild-type mice at the same
time points of wild-type.