Figure 7 of Mitra, Mol Vis 2013; 19:1029-1038.


Figure 7. Flow cytometry analysis showing the uptake of siRNA loaded AuNP-PEI/AuNP-PEI EpAb nanoparticles by Y79 cells. (A) Control untreated Y79 cells run as control (violet area-M1); (B) Y79 cells treated with naked siRNA shows only 0.2% naked siRNA uptake (green peak-M2) when compared to normal untreated Y79 cells (violet area); (C) Y79 cells treated with AuNP-PEI-siRNA shows 29.2% siRNA uptake (green peak-M2) when compared to normal untreated Y79 cells (violet area); (D) Y79 cells treated with AuNP-PEI-EpAb-siRNA shows 59% of siRNA uptake (green peak-M2) when compared to normal untreated Y79 cells (violet area); (E) Only 0.4% uptake of AuNP-PEI-EpAb nanoparticles was observed in EpCAM-siRNA treated Y79 cells. Flow cytometry analysis demonstrated higher uptake of EpCAM conjugated AuNP-PEI-siRNA nanoparticles (59%) by Y79 cells when compared to EpCAM unconjugated AuNP-PEIsiRNA (29.2%; p<0.05).