Figure 1. Co-localization and interaction between Pax6 and TGF-β. Fluorescence microscopy images of the retinal region of murine eyes sections showing co-localization and interaction of Pax6 and TGF-β. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. The sections were immunostained with anti-Pax6+anti–transforming growth factor–β (TGF-β). A: The green signal, was detected by fluorescein isothiocynate (FITC)-conjugated secondary IgG that shows the expression of Pax6, B: the red signals detected by tetra-methyl rhodamine isothiocynate (TRITC)-conjugated secondary IgG show the expression of TGF-β. C: Merged images show the co-localization of Pax6+TGF-β. Arrows in the insets (D, E, F) show the magnified images of the expression and co-localization. Negative control for immunostaining. G: without anti-Pax6 primary antibody but with FITC-conjugated secondary antibody no signals were observed, H: without anti-TGF-β primary antibody but with TRITC-conjugated secondary antibody no signals were observed, I: blue signal by staining with DAPI shows the entire population of the cells. J: Co-immunoprecipitation assay between Pax6 and TGF-β that is immunoprecipitated (IP) with anti-Pax6 and immunoblotted (IB) with anti-TGF- β. The next lane of the blot shows negative control of IP experiment with protein-G slurry without anti-Pax6 antibody, K: Immunoblotting (IB) with anti-Pax6 for immunoprecipitated (IP) with anti-TGF-β that confirms their physical interaction. The next lane of the blot shows negative control of IP experiment with protein-G slurry without anti-TGF-β antibody.