Table 2 of
Hambright, Mol Vis 2012; 18:920-936. Table 2 of
Hambright, Mol Vis 2012; 18:920-936.
Table 2. Primary antibodies.
| Target phenotypes | Target proteins/ Epitopes | Host | Dilution | Vendor |
|---|---|---|---|---|
| Primary antibodies | ||||
| Neural stem cell and/or precursor | Nestin (human, not rat/mouse-specific) | Mouse | 1:400 | Millipore |
| Neuronal precursor | Doublecortin (DCX) | Guinea pig | 1:3,000 | Millipore |
| Neuron - early | Type III b-Tubulin epitope J1 (Tuj1) | Rabbit | 1:1,000 | Covance |
| Neuronal precursor | Doublecortin-like kinase (DCAMKL1) | Rabbit | 1:50 | Dr. Walsh, Harvard |
| Mitotic marker | Ki67 antigen (NCL-Ki67p), Kiel University -raised antibody # 67 | Rabbit | 1:1,000 | Novocastra Labs |
| Human Nuclei -all | Human nuclear protein epitope (HNu) | Mouse | 1:1,200 | Millipore |
| Neuron -human | Synaptophysin, human, not rat/ mouse –specific(Syn) | Mouse | 1:800 | Millipore |
| Muller glia -activated | GFAP | Rabbit | 1:700 | DAKO |
| Photoreceptor | Recoverin (Rec) | Rabbit | 1:1,000 | Millipore |
| Rod photoreceptor | Rhodopsin, Rho4D2 | Mouse | 1:100 | Dr.Molday (UBC) |
| Microglia | Ionized calcium binding adaptor molecule-1 (Iba-1) | Rabbit | 1:200 | Waco Chemicals USA |
| Secondary antibodies | ||||
| Alexa Flour 488 | Goat anti-Rabbit | Goat | 1:400 | Invitrogen |
| Alexa Flour 594 | Goat anti-Mouse | Rabbit | 1:400 | Invitrogen |