Figure 8. Role of p38 activation in
induction of heme oxygenase-1 (HO-1) by curcumin. A:
Retinal pigment epithelium (RPE) cells were treated with 15 μM
curcumin at the indicated time point. Phosphorylated p38 and p38
levels were detected with western blotting. B: RPE cells
were pretreated with 15 µM curcumin and 10 µM CoPP for 3 h.
About 10 µM ZnPP or 20 µM SB203580 as a p38 inhibitor was added
to cells preprocessed for 1 h after 2 h pretreatment with 15 µM
curcumin. Cells were then treated with 1000 µM H2O2
for 3 h. Cell viability was determined with
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT) assay. Data are expressed as mean±SEM from four
independent experiments. *p<0.05, **p<0.01, ***p<0.001.