Figure 7. Enzyme-Linked ImmunoSpot (ELISpot) assay of interferon (INF) -γ in single T cell suspensions from dispase-injected and saline-injected mouse spleens. A: Compared with positive control group (stimulated with phytohemagglutinin, PHA), IFN-γ values were only detected at 4-week and 8-week time points in dispase-injected mice, and not detected at any time point in saline-injected mice. B: In the graph of counting statistics, there were only 25.5 points at the 4-week time point, and 2.5 points in the 8-week for dispase-injected mice.