Figure 5. Immunofluorescence analysis showed alpha smooth muscle actin (α-SMA; A), glial acidic fibrillary protein (GFAP; B), glutamine synthase (GS; E), and retinal pigment epithelia Protein 65 (RPE-65; F) in epiretinal membranes (EMs) of proliferative vitreoretinopathy (PVR) model eyes, indicating fibroblast cells, Müller cells, astroglial cells, and RPE cells involved in the process of PVR. Hoechst 33342 for nucleic acid stained alone (C, G). D is the merged picture of A-C, H the merged picture of E-G (a triple staining). Arrow shows EM.