Figure 5 of Winkler, Mol Vis 2012; 18:874-886.

Figure 5. siRNA decreased transforming growth factor-β1 (TGF-β)-induced connective tissue growth factor (CTGF) in Rat-2 fibroblasts. CTGF mRNA expression in Rat-2 cells treated with TGF-β and siRNA were analyzed using real-time PCR and normalized to the housekeeping gene acidic ribosomal phosphoprotein P0 (ARPP0). A: The effect of two separate CTGF siRNAs (I and III) and a control scrambled siRNA on TGF β-induced CTGF expression in Rat-2 fibroblasts was tested. Real-time PCR showed that siRNA I and siRNA III decreased CTGF mRNA by 75% and 86%, respectively. B: Arepresentative western blot documenting that TGF-β induced CTGF protein decreased by siRNA I and III. Proteins were run on the same gel, but not in adjacent lanes. C: Densitometric analysis summarizing the results of three separate western blots shows that siRNA I and siRNA III decreased CTGF protein by 49% and 46%, respectively. (*p<0.05). n=3/group.