Figure 3. Connective tissue growth factor (CTGF) was detected in vimentin-positive Müller cells in the retina of diabetic rats. A: Müller cells labeled with filament protein vimentin throughout the diabetic retina. B: CTGF staining in the 12-week diabetic retina is also seen throughout the retina. C: Merge photomicrograph for vimentin and CTGF shows that CTGF colocalizes with vimentin in the diabetic retina. Bar=60 um. D: High magnification of the area indicated with a dotted rectangle in C. Bar=5 um. V=Vitreous, ONL=Outer Nuclear Layer, S=Sclera.