Figure 1 of Winkler, Mol Vis 2012; 18:874-886.


Figure 1. Connective tissue growth factor (CTGF) expression increased in retina of 8 and 12 week diabetic rats. CTGF mRNA expression in the retinas of non-diabetic and diabetic rats after 8 and 12 weeks of hyperglycemia were analyzed using real-time PCR and normalized to the housekeeping gene acidic ribosomal phosphoprotein P0 (ARPP0). A: CTGF mRNA levels increased six- and sevenfold at both time points. B: A representative western blot illustrating CTGF and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression in the retinas of diabetic rats after 8 and 12 weeks of hyperglycemia. Note the increased CTGF protein levels compared to the non-diabetic controls. C: Densitometric analysis of three separate immunoblots indicates a 2.5 and 5.7 fold increase in the CTGF protein after 8 and 12 weeks of hyperglycemia, respectively. (*p<0.05, **p<0.001 diabetic versus non-diabetic) n=non-diabetic; D=diabetic. Proteins ran on a 4%–15% gradient gel. n=3/age.