Figure 6. Effects of HIF-1α shRNA on HIF-1α, VEGF, MMP-2/9, and IL-1β expression in corneas with CL. HIF-1α and VEGF were detected by RT–PCR (A) and western blotting (C) while MMP-2/9 and IL-1β were detected by RT–PCR (A). GAPDH was used as the internal control in all cases. As shown by the analysis results (B, D) compared with the control group, HIF-1α RNAi-A significantly down-regulated the expression levels of VEGF, MMP-2/9, and IL-1β. Three independent experiments were conducted, and data were shown as mean±SD *p<0.05, **p<0.01 as compared with control group.