Figure 4. Inhibition of HIF-1α induced by CoCl₂ by HIF-1α specific shRNA in HCECs. A: HIF-1α was upregulated after 3 h and 5 h induction of CoCl₂ over normal corneas. B: Quantitative analysis of HIF-1α protein expression in HCECs. Results were mean±SD, n=3, **p<0.01 as compared with 0 h group. C: western blotting analysis of HIF-1α expression in HCECs treated with shRNA targeting HIF-1α (RNAi-A, RNAi-B) and nonspecific shRNA (Vehicle- RNAi) and incubated with CoCl₂. D: Quantitative analysis of HIF-1α expression from panel C. Results are mean±SD, n=3, *p<0.05, **p<0.01 as compared with CoCl₂ group.