Figure 9. Phalloidin staining
(magnification 400×) showing the disorganization of F-actin
cytoskeleton in HO90 mM condition (B) compared to DMEM
control (A). BAK10−4%-stress (C),
BAK3.10−4% (D), and BAK5.10−4% (E)
induced cell blebs, cytoplasm shrinkage, and cell detachment in
a dose-dependent manner. All BAK concentrations induced more
disorganization and cell shrinkage than even the highest
hyperosmolar stress. Combinations of both stresses, namely HO90
mM+BAK10−4% (F), HO90 mM+BAK3.10−4%
(G), and HO90 mM+BAK5.10−4% (H) deeply
altered cell morphology, such as cytoplasm retraction associated
with cell-size decrease (large arrows), and induced a
dose-dependent cell death characterized by the presence of
apoptotic bodies (thin arrows).
Figure 9
of Clouzeau, Mol Vis 2012; 18:851-863.