Figure 1. Phase contrast imaging of WKD live cells in DMEM (t=0 s) stressed at t=2 s by complementing media to obtain final concentration of HO90 mM (A; animation1), BAK10⁻²% (B: animation 2), and HO90 mM+BAK10⁻²% (C; animation3). First line: HO90 mM at t=0 s, t=5 s, and t=60 s; HO90 mM stress induced a cell-volume decrease for 3 s after stress observed at t=5 s before returning to almost normal size ( t=31 s), then a very discreet cytoplasmic retraction was observed. Second line: BAK10⁻²% at t=0 s, t=60 s, and t=96s ; BAK induced a progressive cytoplasm shrinkage observed from t=3 s to t=60 s (circles) and followed by intense cell blebbing (arrows, from t=60 s) before cell death. Last line: HO90 mM+BAK10⁻²% at t=0 s, t=7 s, and t=31 s. Cells exhibited profound alteration just after stress, such as disruption of plasma membrane and nuclear fragmentation leading to irreversible cell death already visible at t=7 s.