Figure 6. Western blot analysis of cytochrome c and cleaved caspase-3 protein. At 48 h after transfection, the cells were harvested and processed for western blotting. β-Actin was used as the internal control. The immunoblot was a representative of three independent experiments. The expression of cytochrome c and the activity of cleaved caspase-3 were greatly improved (p<0.05) in the PAMAM group and liposome group, compared with the negative control group and the empty control group.