Figure 5. Bcl-2 shRNA induced HLECs apoptosis. Apoptosis was observed by Hoechst 33258 staining (original magnification 40×). After human lens epithelial cells (HLECs) were transfected for 48 h, marked morphological changes were clearly seen on Hoechst 33258 staining in the PAMAM group (A) and the liposome group (B), compared with the negative control group (C) and the empty control group (D). The changes were suggestive of apoptosis, including condensation of chromatin, nuclear fragmentations, and apoptotic bodies. Scale bar, 20 µm.