Table 1 of
Weisschuh, Mol Vis 2012; 18:751-757. Table 1 of
Weisschuh, Mol Vis 2012; 18:751-757.
Table 1. Primer sequences for PCR amplification of PAX6.
Primer name |
Primer sequence |
Annealing temperature (°C) |
Amplicon size (bp) |
|---|---|---|---|
| PAX6-Exon4-f | tgtaggggaaacaga | 55 | 491 |
| PAX6-Exon4-r | atcgagaagagccaagcaaa | ||
| PAX6-Exon5-f | ctggtggtcctgttgtcctt | 55 | 446 |
| PAX6-Exon5-r | atgaagagagggcgttgaga | ||
| PAX6-Exon6-f | gggctacaaatgtaattttaagaa | 55 | 509 |
| PAX6-Exon6-r | agagagggtgggaggaggta | ||
| PAX6-Exon7-f | gagctgagatgggtgactg | 55 | 300 |
| PAX6-Exon7-r | gagagtaggggacaggcaaa | ||
| PAX6-Exon8-f | tcaggtaactaacatcgca | 55 | 719 |
| PAX6-Exon8-r | gttgactgtacttggaagaa | ||
| PAX6-Exon9-f | aggtgggaaccagtttgatg | 55 | 311 |
| PAX6-Exon9-r | catggcagcagagcatttag | ||
| PAX6-Exon10-f | gctaaatgctctgctgccat | 55 | 329 |
| PAX6-Exon10-r | agagtgagagtcagagcccg | ||
| PAX6-Exon11-f | ccgggctctgactctcact | 55 | 221 |
| PAX6-Exon11-r | gccactcctcacttctctgg | ||
| PAX6-Exon12-f | gaggcttgatacataggc | 55 | 452 |
| PAX6-Exon12-r | ccataagaccaggagatt | ||
| PAX6-Exon13-f | tccatgtctgtttctcaaagg | 55 | 220 |
| PAX6-Exon13-r | tcaactgttgtgtccccatag |