Figure 5. Sequence analysis of RT–PCR products. RNA was derived from HEK293 cells transfected with the wildtype c.1183+5 G-allele plasmid construct (A) and the mutant c.1183+5 A-allele plasmid construct (B). A: Red letters indicate the tat exon 1 sequence and black letters indicate sequence of PAX6 exon 12. B: Red letters indicate the tat exon 1 sequence and green letters indicate the tat exon 2 sequence.