Figure 5. In mice, phototoxicity does not induce vascular leakage. Retinal whole mounts of the left retinas showing the retinal vessels. These animals had received an intravenous horseradish peroxidase (HRP) injection before processing and their retinas were processed for HRP demonstration (Hanker-Yates method). A: control mouse, B-D: photoexposed mice processed at increasing times ALE. B: 1 day, C: 7 days, D: 30 days ALE. Although in some cases a few areas of discrete HRP leakage around the vessels due to retinal damage during dissection are observed, a diffuse HRP staining, indicative of vascular leakage, was never detected. Abbreviations: S: superior, I: inferior, N: nasal, T: temporal.