Molecular Vision: Ha, Mol Vis 2012; 18:645-656. Table 3

Table 3. Summary of candidate Wnt target gene validation.

				qPCR validation
Gene	Fold-change on microarray	# TCF binding sites	# conserved sites	Explants	β-cat^Act	in situ β-cat^Act
Wnt pathway
Wif1	29.2	7	1	+	+	+
Axin2	2.8	6	4	+	+	nd
Ciliary margin markers
Msx1	2.3	6	3	nd	nd	+
Otx1	1.8	9	7	nd	nd	+
Neuron development
Epha2	8.4	4	1	-	-	nd
Efna3	1.7	4	1	+	-	nd
Syt13	−11	6	0	+	-	+
Prickle1	−6	9	1	+	-	nd
Ndrg2	−2.8	4	2	+	-	nd
Cell cycle
Cdkn1a	21.9*	3	0	+	+	+
Differentiation
Serpine2	25.1	1	1	+	-	nd
Klf10	2.3	1	0	+	-	nd
Cdc25b	1.9	4	1	+	-	nd
Lix1	2.5**	12	4	-	+	-
Signaling
Tnfrsf19	20.9	10	2	+	+	+
Med12	2	8	0	-	-	-

Gene expression was compared by q-RT–PCR analysis in [1] Li+-treated and untreated retinal explants [2]; whole retinas of E14 β-cat^Act and control littermates and by in situ hybridization in retina sections from β-cat^Act and control littermates at E14. (+) indicates that the expression of the gene exhibited the same trend (either up or down) as reported in the microarray analysis. (-) no change in the gene expression was detected or the change in expression was opposite to that reported in the microarray analysis. nd, not done. Asterisks indicate that the reported fold-change is the average value obtained from two (*) or three (**) different probes on the microarray.