Figure 3. Flow cytometric analysis of the T cell phenotype in the right cervical lymph node, peripheral blood, and spleen. A: Flow cytometry of the spleen of control mouse 2 on postoperative day 14. Gate 1: lymphocytes (R1, indicated in red). B: CD4+T cells (R2. Indicated in green) in lymphocytes. C: Double labeling CD25+/Foxp3+ lymphocytes in CD4+ T population. Gate 2: R1 and R2.