Table 1 of
Xiang, Mol Vis 2012; 18:617-623. Table 1 of
Xiang, Mol Vis 2012; 18:617-623.
Table 1. Primers used in polymerase chain reaction for amplification of NOD2/CARD15.
Exon |
Annealing temperature (°C) |
Primer direction |
Sequence (5′→3′) |
Amplicon size (bp) |
|---|---|---|---|---|
| 1 | 55.0 | Forward | GTAGACAGATCCAGGCTCAC | 384 |
| Reverse | ACCAGCCAAGGATGCGACAG | |||
| 2 | 55.0 | Forward | TGACCACCCTGCATCTGGCTT | 627 |
| Reverse | ACCAAGTTACCCCACAGGCTG | |||
| 3 | 55.0 | Forward | CAGTAAGCCTTCCCACATTG | 451 |
| Reverse | AACACCTTTAGTTAGCCCTCA | |||
| 4a | 55.0 | Forward | CTGGCTCTCCTATCCCTTCA | 555 |
| Reverse | TGTCTTCTTGACCAACATCAG | |||
| 4b | 55.0 | Forward | TCTCTTTGTCTTCCCATTCAG | 462 |
| Reverse | AGGGCTGAGGTCTCTTGGA | |||
| 4c | 55.0 | Forward | GCTTCTCTGAACAGGGCATC | 689 |
| Reverse | TGCTGTGATCTGAAGGTTGTG | |||
| 4d | 55.0 | Forward | AGGTGTCGTGCCAGGGAGTA | 755 |
| Reverse | CACACTTAGCCTTGATGGTG | |||
| 5,6 | 55.0 | Forward | GCACAGATGCTGGCACTTC | 565 |
| Reverse | CAGATCAGACTGACTCAGGAAT | |||
| 7 | 55.0 | Forward | GTAAACTAGACCTAGCAGCGA | 277 |
| Reverse | CTCCATGCAGGTCCCTCTTC | |||
| 8 | 55.0 | Forward | GGAGGAGGACTGTTAGTTCAT | 362 |
| Reverse | AGAGGACAAGGGACATTTCCA | |||
| 9 | 55.0 | Forward | AGACCAGGAGAGCACCACGA | 301 |
| Reverse | CAGTCAATCACTCAATCATCCA | |||
| 10 | 55.0 | Forward | TGTGAGTTCATCATCTTCCATA | 297 |
| Reverse | ATCCTTGTCCACCTAGACCA | |||
| 11 | 55.0 | Forward | CTCATTGGGAATCTCAGACAT | 391 |
| Reverse | CAGAGAATCAGATCCTTCACAT | |||
| 12 | 55.0 | Forward | GAGAGTCAGCCCATCCCAG | 476 |
| Reverse | AGCAGAGGCCAGTCCCATACT |