Figure 5. Recruitment of inflammatory cells to the cornea of Dstn mutants. A: Immunofluorescence using CD45 (red) to mark inflammatory cells and myeloperoxidase to mark neutrophils specifically in Dstn mutant and WT cornea on A.BY and B6 backgrounds at P58. All slides were counterstained with DAPI to mark cell nuclei (blue). Bar, 20 μm. B: Quantification of CD45 positive cells revealed significantly increased inflammation in both Dstn^corn1 and Dstn^corn1–2J mutant cornea compared to WT at P58 (p<0.001 for both backgrounds). C: The trend for increased inflammation in A.BY Dstn^corn1 compared to B6.Cg-Dstn^corn1 is present by P28 and increases to significance by P58. Sample sizes: A.BY.Cg-Dstn^corn1-2J P58 n=4, A.BY Dstn^corn1 P58 n=8, A.BY WT P58 n=4, B6 Dstn^corn1–2J P58 n=4, B6.Cg-Dstn^corn1 P58 n=6, B6 WT P58 n=4, A.BY Dstn^corn1 P28 n=6, B6.Cg-Dstn^corn1 P28 n=3. Unit length=300 um. Error bars, SEM * denotes statistical significance resulting from t-tests, with omitted bars representing non-significance. *p<0.05, **p<0.01, ***p<0.001.