**Figure 5. **Recruitment of inflammatory
cells to the cornea of *Dstn* mutants. **A**:
Immunofluorescence using CD45 (red) to mark inflammatory cells
and myeloperoxidase to mark neutrophils specifically in *Dstn*
mutant and WT cornea on A.BY and B6 backgrounds at P58. All
slides were counterstained with DAPI to mark cell nuclei (blue).
Bar, 20 μm. **B**: Quantification of CD45 positive cells
revealed significantly increased inflammation in both *Dstn*^{corn1}
and *Dstn*^{corn1–2J} mutant cornea compared to WT
at P58 (p<0.001 for both backgrounds). **C**: The trend
for increased inflammation in A.BY *Dstn*^{corn1}
compared to B6.Cg-*Dstn*^{corn1} is present by P28
and increases to significance by P58. Sample sizes: A.BY.Cg-*Dstn*^{corn1-2J}
P58 n=4, A.BY *Dstn*^{corn1} P58 n=8, A.BY WT P58
n=4, B6 *Dstn*^{corn1–2J} P58 n=4, B6.Cg-*Dstn*^{corn1}
P58 n=6, B6 WT P58 n=4, A.BY *Dstn*^{corn1} P28
n=6, B6.Cg-*Dstn*^{corn1} P28 n=3. Unit length=300
um. Error bars, SEM * denotes statistical significance resulting
from *t*-tests, with omitted bars representing
non-significance. *p<0.05, **p<0.01, ***p<0.001.