Figure 4 of Kawakami-Schulz, Mol Vis 2012; 18:606-616.

Figure 4. Epithelial hyperproliferation in Dstncorn1 corneas. A: Immunofluorescence for Ki67 (green) labels proliferating cells in the corneal epithelium of Dstn mutants and WT mice on A.BY and B6 backgrounds at P58. All slides were counterstained with DAPI to mark cell nuclei (blue), which is shown merged with Ki67 staining in the lower panels. Bar, 20 μm. B: Quantification of Ki67 positive cells shows significantly increased numbers of proliferating cells in Dstncorn1 cornea compared to Dstncorn1–2J and WT cornea in both A.BY and B6 backgrounds at P58 (p<0.001 for both backgrounds). C: A tendency for increased hyperproliferation in A.BY Dstncorn1 compared to B6.Cg-Dstncorn1 is observed by P28. This difference becomes statistically significant by P58. Sample sizes: A.BY.Cg-Dstncorn1–2J P58 n=4, A.BY Dstncorn1 P58 n=5, A.BY WT P58 n=4, B6 Dstncorn1–2J P58 n=3, B6.Cg-Dstncorn1 P58 n=5, B6 WT P58 n=4, A.BY Dstncorn1 P28 n=6, B6.Cg-Dstncorn1 P28 n=3. Unit length=300 μm. Error bars, SEM * denotes statistical significance resulting from t-tests, with omitted bars representing non-significance. *p<0.05, **p<0.01, ***p<0.001.