Figure 3. Corneal neovascularization caused by the Dstn^corn1 mutation. A: Immunofluorescence for CD31 highlights blood vessels that infiltrate Dstn^corn1 cornea in both the A.BY and B6 background. Significant neovascularization is not observed as a result of the Dstn^corn1–2J mutation, making the appearance similar to WT. Bar, 200 μm. B: Quantification of the vascularized area shows that Dstn^corn1 cornea have significantly more vasculature compared to Dstn^corn1–2J and WT cornea in both genetic backgrounds (p<0.001 for both backgrounds). Note that the resting level of vasculature is higher in B6 than A.BY. C: Genetic background effect on the neovascularization phenotype in the Dstn^corn1 cornea is significant at postnatal day 28, and becomes even more significant with age. Sample sizes: A.BY.Cg-Dstn^corn1–2J P58 n=5, A.BY Dstn^corn1 P58 n=11, A.BY WT P58 n=3, B6 Dstn^corn1–2J P58 n=4, B6.Cg-Dstn^corn1 P58 n=10, B6 WT P58 n=4, A.BY Dstn^corn1 P28 n=11, B6.Cg-Dstn^corn1 P28 n=6. Error bars, SEM * denotes statistical significance resulting from t-tests, with omitted bars representing nonsignficance. *p<0.05, **p<0.01, ***p<0.001.