Figure 2. F-actin in the cornea of Dstn mutants and WT mice in both the A.BY and B6 background at P58. A: Phalloidin staining of whole mount cornea at P58 shows that F-actin (red) accumulation more severe in Dstn^corn1 cornea compared to Dstn^corn1–2J cornea in both the A.BY and B6 background. F-actin accumulation is less severe in the cornea of B6.Cg-Dstn^corn1 when compared to A.BY Dstn^corn1 . Bar, 200 μm. B: Phalloidin staining of frozen sections illustrates the degree of F-actin (gray in upper panels, red in lower panels) accumulation in each Dstn mutant compared to WT. Two portions of an A.BY Dstn^corn1 cornea are shown to represent its irregular surface. Bar, 20 μm. All slides were counterstained with DAPI to mark cell nuclei (blue).