Figure 8. Effect of 3 culture media on UCB1 MSC association with damaged endothelium. Fluorescence confocal microscopic images show the formation of MSC cell sheets in areas of damaged HCEC (arrows in A-C). Note that, in wounded corneas incubated in MSCBM, ZO1 is localized diffusely within the cytoplasm of the MSCs. In wounded corneas incubated in LECBM or LECCM, ZO1 tended to be localized at the lateral borders of MSCs. Red: ZO1. Blue: TO-PRO-3-stained nuclei. Original magnification: 40×.