Figure 7. Attachment of GFP-labeled UCB1 MSCs to damaged endothelium. The image in (A) shows lack of attachment of UCB1 MSCs to unwounded endothelium. The inset shows results of the no-primary negative control for ZO1 staining. GFP-labeled UCB1 MSCs in (B) attached to damaged endothelium in the crush wound model. Arrowheads indicate the ZO1 pattern of unwounded HCEC. The image in (C) shows attachment of GFP-labeled UCB1 MSCs to remnants of damaged endothelium in the scrape wound model. Arrows indicate areas of damaged HCEC. Red: ZO1. Blue: TO-PRO-3-stained nuclei. Blue: TO-PRO-3-stained nuclei. Original magnification: 40×.