Figure 6. Effect of EGTA treatment on UCB1 MSC morphology and junction-associated protein localization. Top four phase-contrast images demonstrate that EGTA treatment induces separation of UCB1 MSCs in cultures grown in either lens epithelial cell basal medium (LECBM) or lens epithelial cell-conditioned medium (LECCM). Arrows in the (+) EGTA images indicate large spaces between cells. Confocal fluorescence images at the bottom demonstrate changes in the relative localization of N-cadherin (FITC) and ZO1 (rhodamine) in UCB1 MSCs grown in LECCM. Both bottom images are overlays with TO-PRO-3 (blue) to visualize nuclei. Phase contrast original magnification: 4×. Confocal original magnification: 40×.