Figure 5. ZO1 (red) and N-cadherin (green) staining patterns in UCB1 MSCs incubated in three different culture media. Images in A-C show ZO1 staining alone, while images D-F show an overlay of the ZO1 and TO-PRO-3 (blue) staining, so individual cells can be observed. Images G-I show N-cadherin staining alone, while images J-L show an overlay of the N-cadherin and TO-PRO-3 staining. Images M and N are negative controls showing overlays of the rhodamine and TO-PRO-3 channels (M), and FITC and TO-PRO-3 channels (N). Original magnification: 40×.