Figure 6 of Reinisalo, Mol Vis 2012; 18:38-54.


Figure 6. Modulation of OTX2 levels in RPE cells results in parallel expression of tyrosinase mRNA. A: Various amounts (0 to 1.5 µg) of OTX2 expression plasmid were transfected into D407 cells followed by measurement of tyrosinase mRNA expression. Results are presented as the mean normalized expression±SEM relative to maximal OTX2 expression plasmid amount (1.5 µg=100) from three independent transfections each performed in triplicate. B: Endogenous tyrosinase, OTX2, and MITF-A mRNA levels were determined 48 h after transfection of OTX2- or MITF-specific siRNA into ARPE-19 cells. Results are presented as the mean normalized expression±SD relative to cells transfected with non-specific siRNA (=100) from three independent transfections each performed in triplicate.