Figure 6. Modulation of OTX2 levels
in RPE cells results in parallel expression of tyrosinase mRNA.
A: Various amounts (0 to 1.5 µg) of OTX2 expression
plasmid were transfected into D407 cells followed by measurement
of tyrosinase mRNA expression. Results are presented as the mean
normalized expression±SEM relative to maximal OTX2 expression
plasmid amount (1.5 µg=100) from three independent transfections
each performed in triplicate. B: Endogenous tyrosinase,
OTX2, and MITF-A mRNA levels were determined 48 h after
transfection of OTX2- or MITF-specific siRNA into ARPE-19 cells.
Results are presented as the mean normalized expression±SD
relative to cells transfected with non-specific siRNA (=100)
from three independent transfections each performed in
triplicate.