Figure 3 of Reinisalo, Mol Vis 2012; 18:38-54.

Figure 3. OTX2 can activate the human TYR promoter even in the absence of DNA binding sites. OTX2 directly activates the TYR promoter through specific OTX2 binding sites 1 (−361 to −356), 2 (−354 to −349) and 3 (−226 to −221) and indirectly via MITF sites. A: Luc-reporter constructs containing disrupted MITF binding sites at positions −104 to −99 (mM=mutation of the M box) and −12 to −7 (mE=mutation of the E box). B: Disruptions of OTX2 binding sites 1, 2, and/or 3 (renamed m1, m2, and m3) in the human TYR promoters −152 or −462 were cotransfected into ARPE-19 cells with MITF-A or OTX2 factors, their combination, or with an empty expression vector (pCR3). Normalized luciferase activities are relative to TYR promoter construct −152 (=1) cotransfected with the empty expression vector pCR3 (=vector only). Data are means±SEM from two independent transfections each performed in triplicate.